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This volume brings together critical insights into the biology, epidemiology, and diagnostics of major parasitic threats to both veterinary and human health. Chapter One explores the complex world of equine cyathostomins, addressing their lifecycle, clinical manifestations such as acute larval cyathostominosis, immune interactions, and the challenges posed by widespread anthelmintic resistance. Building upon the necessity for precise diagnostic tools, Chapter Two shifts the focus to human health, re-evaluating soil-transmitted helminth diagnostics in the genomic era. The authors highlight how unaddressed genetic diversity in diagnostic target regions can compromise traditional and molecular tools like qPCR, risking "e;diagnostic escape,"e; and advocate for integrating whole-genome sequencing and nemabiome approaches into public health surveillance. Finally, Chapter Three provides a comprehensive, One Health-driven synthesis on zoonotic sarcocystosis, addressing how Sarcocystis species cross ecological boundaries between wildlife, livestock, companion animals, and humans.* Biology, diagnosis and management of equine cyathostominosis Soil-transmitted helminth diagnostics in the post-genomics era Zoonotic pathways and host dynamics