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This volume§presents detailed laboratory protocols regarding the three major route§technologies luciferases, efficient optical§probes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology series§format, chapters include introductions to their respective topics, lists of the§necessary materials and reagents, step-by-step, readily reproducible laboratory§protocols, and tips on troubleshooting and avoiding known pitfalls.§§Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a useful§complement to the first and second edition for new and experienced researchers§alike.§§
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