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Computational analysis of week interactions in TIM-Barrel Proteins describe in detail the occurrence, secondary structural preference and other biophysical parameters required for the formation and stabilization of non-canonical week interactions in TIM-barrel Proteins. This book also explores the significance of non-canonical week interactions through mutating the amino acids in selected TIM-barrel proteins. This comparative study on week interactions in native and mutant TIM-barrel proteins was explored through molecular dynamic simulation studies.