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As in the previous three editions, the intent of this fourth edition is to summarize the advances of the past decade on the structure, mechanism, and biochemistry of cytochrome P450 enzymes, but with sufficient coverage of earlier work to make each chapter a comprehensive review of the field. In broad terms, the first five chapters of this book deal with the biophysics and mechanistic enzymology of cytochrome P450 enzymes, with a discussion of the structures of P450 enzymes and their electron donor partners, the mechanisms of oxygen activation and substrate oxidation, and the approaches and nature of cytochrome P450 inhibition. The subsequent section of the book focuses on the roles of P450 enzymes in biology, starting with three extensive chapters that review the nature and roles of cytochrome P450 enzymes in humans, microbes, plants, and insects. Four chapters then deal with the regulation of P450 enzymes and their roles in the processing of sterols and lipids. The book closes with a survey of the potential utility of P450 enzymes in biotechnology.
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