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Development of a CellChip based Multi-Well-Type Bioreactor
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In this work a novel multi-purpose platform for the §three-dimensional cultivation of cells is described, §its main component being a polymer chip (CellChip) §that features a microstructured area of 1-2cm2.§The microstructured area is realized as a grid of §micro-containers measuring 300µm in depth, length §and height. Depending on the cells and the chip type §used up to 7 106 cells can be cultivated on one chip.§For the facilitation of nutrient and oxygen supply §the CellChip can be inserted into a bioreactor §housing that can be tailored to the specific §application. For drug screening and other high-§throughput purposes a bioreactor with microtiter §plate-footprint was developed in this work. Up to 12 §CellChips can be incorporated into this bioreactor §with independent nutrient and oxygen supply.
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