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This book constitutes the proceedings of the 25th International Conference on Information Processing in Medical Imaging, IPMI 2017, held at the Appalachian State University, Boon, NC, USA, in June 2017. The 53 full papers presented in this volume were carefully reviewed and selected from 147 submissions. They were organized in topical sections named: analysis on manifolds; shape analysis; disease diagnosis/progression; brain networks an connectivity; diffusion imaging; quantitative imaging; imaging genomics; image registration; segmentation; general image analysis.