Insect Genomics

This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book is also meant to provide an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover topics such as determining genome size by fluorescent measurement in a flow starter; HMW DNA extraction; improvements to a genome project provided by long range sequencing approaches; assessments of single copy genes at different phylogenetic levels; FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; and a look at ways to examine insect genomes (TEEseq) for the presence of endosymbionts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.