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Microfluidic Technologies for Single-Cell Analysis
Creating uniform cellular microenvironments for fast timescale measurements
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Analysis of single cells allows a complete§understanding of the heterogeneity that is present in§cell behavior and function. Current single-cell§analysis methods provide high-throughput information§about labeled biomolecules within cells, but often§cannot follow the dynamic processes occurring in§signaling pathways. New microfluidic methods have§separately allowed assays of fast timescale responses§or creation of uniform environments to study cell§behavior in a more quantitative manner. This book§focuses on microfluidic hydrodynamic trapping§techniques that aid in both fast timescale§measurements and uniform environmental control in a§single platform. Example applications for fast§timescale analysis of pore-forming toxin insertion§into membranes and assays of single-cell enzyme§content will be presented. The book would be of§interest to analytical chemists, engineers, and§biologists developing platforms to study single-cell§behavior. Also, biologists looking for tools to§study cells of interest with statistical accuracy§would find the book most helpful.
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