Stem Cell Laboratory Techniques

Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications, used by researchers around the world. The guide begins with the introduction of stem cells, including definitions, types, and basic use of stem cells in biomedical science research. As a guide book about stem cell lab techniques, this book explains the laboratory procedures and techniques ranging from extraction of stem cells from animal, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording and quality control, to differentiation techniques for confirmation studies. For the analysis of stem cell study, the book guides researchers in the topics of transcriptome analysis, proliferation study analysis, and microphysiological study. Stem Cell Laboratory Techniques also includes the useful and recent applications in stem cells, such as gene editing techniques for stem cell, and preparation of stem cells for in vitro study, as well as the stem cell lab design and equipment used in the lab. Lastly, human and animal research ethics will be discussed. Because the book covers the basics, which is required in every stem cell laboratory, through the updated application of stem cells it is a must read for anyone researching or studying stem cells. Introduces readers to the stem cell culture and moves to handling techniques and versatile applications Includes coverage of gene editing techniques for stem cells and stem cells for in vitro study Presents stem cell lab design and equipment used in the lab