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Techniques to Investigate Mitochondrial Function in Neurons

Stefan Strack

Yuriy M. Usachev

Lingua

Inglese

Libro Rigido

Codice Libristo: 15530039

Casa editrice Humana Press Inc., marzo 2017

This volume describes a broad spectrum of experimental approaches for investigating structure, funct... Descrizione completa

Codice Libristo: 15530039

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This volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease. Most of these approaches were only recently developed and range from electron tomography-based 3D reconstruction of mitochondrial cristae to patch clamp recording from mitochondria in intact neurons. The chapters in this book cover topics such as mitochondrial proteomics, fluorescence lifetime imaging, respirometry and mitophagy, as well as optical approaches based on the use of genetically engineered fluorescent sensors for monitoring synaptic ATP and axonal ROS generation, mitochondrial Ca2+ cycling and pH changes, and mitochondrial dynamics and axonal trafficking in live neurons. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Techniques to Investigate Mitochondrial Function in Neurons is a valuable and useful resource for a broad range of investigators interested in the function of neuronal mitochondria in health and disease states.

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Titolo completo Techniques to Investigate Mitochondrial Function in Neurons

Autore Stefan Strack, Yuriy M. Usachev

Lingua

Inglese

Rilegatura Libro - Rigido

Data di pubblicazione 2017

Numero di pagine 344

EAN 9781493968886

ISBN 1493968882

Codice Libristo 15530039

Casa editrice Humana Press Inc.

Peso 824

Dimensioni 185 x 263 x 30

Categorie

Medicina e Infermieristica > Medicina: argomenti di interesse generale > Attrezzature e tecniche mediche > Ricerca medica

Matematica e Scienze > Biologia, scienze della vita > Scienze della vita: argomenti di interesse generale > Neuroscienze

Medicina e Infermieristica > Specializzazioni mediche, branche della medicina > Neurologia e neurofisiologia clinica

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